Journal: Physiological Genomics

Article Title: Mycophenolate mofetil prevents cerebrovascular injury in stroke-prone spontaneously hypertensive rats

doi: 10.1152/physiolgenomics.00110.2016

Figure Lengend Snippet: Renal immune cell infiltration. Immunohistochemistry was used to detect CD43+ lymphocytes (top) and CD68+ macrophages (bottom) infiltration in the kidneys collected from vehicle-treated (−MMF) or MMF-treated (+MMF) rats at the end of the treatment protocol or at the time of euthanasia. Representative images are shown above. Scale bar = 50 μm for CD43 and 20 μm for CD68 images. A large number of infiltrating CD43+ and CD68+ cells (indicated by arrows) were detected in the kidneys of vehicle-treated SHR-A3 rats. Immune cell infiltration was markedly reduced by immunosuppression with MMF for 8 wk; n = 8–9. *P < 0.05 vs. vehicle-treated (−MMF) rats.

Article Snippet: CD68 (clone ED1, ABD Serotec) was used as a marker of activated microglia/macrophages, and CD43 (clone W3/13, ThermoFisher Scientific) was used to identify lymphocytes.

Techniques: Immunohistochemistry

Journal: Frontiers in Immunology

Article Title: Dim Light at Night Impairs Daily Variation of Circulating Immune Cells and Renal Immune Homeostasis

doi: 10.3389/fimmu.2020.614960

Figure Lengend Snippet: Dim light at night (dLAN) disturbs the daily variation and alters the number of circulating immune cells. (A–C) Flow cytometric analysis of peripheral white blood cells (WBCs) collected at ZT9 and ZT21 from rats exposed to either the control light–dark (LD) regime (CTRL) or dLAN (~2 lx) for 2 (upper rows) and 5 (lower rows) weeks. Data represent the mean ± SEM with n = 6–9 per group. (B) Numbers of CD4 + helper (Th) and CD8 + cytotoxic (Tc) T cells and the CD4/CD8 ratio. (C) Numbers of classical (CD43 lo HIS48 hi ) and non-classical (CD43 lo HIS48 hi ) monocytes. Significant differences were evaluated by two-way repeated ANOVA with the Tukey post hoc test for multiple comparisons. Only significant main effects (ZT and dLAN) or interactions (ZT×dLAN) are shown. Dotted lines indicate significant differences between individual groups if an interaction was significant. # P < 0.1, * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The following fluorochrome-conjugated monoclonal antibodies were used: PE-Cy7 anti-rat CD45 (clone OX-1; Sony Biotechnology, USA; 1611065), FITC anti-rat CD3 (clone G4.18; Thermo Fisher Scientific, USA; 11-0030), APC anti-rat CD4 (clone OX-35; Thermo Fisher Scientific; 17-0040), PE anti-rat CD8a (clone OX-8; Thermo Fisher Scientific; 12-0084), PE anti-rat CD45RA (clone OX-33; Thermo Fisher Scientific; MR6404), APC anti-rat CD161a (clone 10/78; Thermo Fisher Scientific; MR6805), FITC anti-rat granulocytes (clone HIS48; Thermo Fisher Scientific; 11-0570), and PE anti-rat CD43 (clone W3/13; Sony Biotechnology; 1614060).

Techniques: Control

Journal:

Article Title: Cyclosporin A reduces expression of adhesion molecules in the kidney of rats with chronic serum sickness

doi: 10.1046/j.1365-2249.2000.01251.x

Figure Lengend Snippet: Leucocyte infiltration in control rats, cyclosporin A (CsA)-treated rats (OVA.CsA) and chronic serum sickness rats (OVA.CSS)

Article Snippet: The following MoAbs were used: anti-CD54, identifies intercellular adhesion molecule-1 (ICAM-1) (Seikagaku Corp., Tokyo, Japan; concentration used 10 μg/ml); anti-CD18, identifies LFA-1β chain (distribution: lymphocytes, monocytes, neutrophils; Sera Laboratories; concentration used 10 μg/ml); anti-CD43, clone W3/13 (distribution: mainly T lymphocytes and to a lesser degree rat thymocytes, neutrophils, plasma cells and stem cells; Accurate Chemical & Scientific Corp., Westbury, MA; concentration used 5 μg/ml); anti-CD11b/c (distribution: monocytes, neutrophils; Biosource Int., Camarillo, CA; concentration used 5 μg/ml); anti-CD45, identifies leucocyte common antigen (LCA) (Biosource Int.

Techniques: